AMP Screening - AIPAMPDS

Screen Peptide Sequences

Screen your peptide sequences using our comprehensive prediction models. The AMP Identifier predicts antimicrobial activity, while the Activity Predictor assesses activity specifically against E. coli and S.aureus. The Hemolysis Predictor evaluates potential hemolytic toxicity. The system supports both FASTA file uploads and direct sequence input. You may input up to 10,000 sequences per submission in the text box, or upload a FASTA file containing up to 10,000 sequences and 5 MB in size.

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Screening includes: AMP identification, antimicrobial activity prediction (E.coli & S.aureus), and hemolysis prediction.

Supported lengths: AMP Identifier 5–298 residues; MIC Classifiers 5–50; Hemolysis Predictor 6–200. Unsupported predictions are N/A. If a required prediction is unavailable, Score is N/A and Evaluate is Incomplete. Input accepts the 20 standard amino acids and X (unknown residue); X is marked with a warning. Other non-whitespace characters are rejected. Warnings and original sequence names are included in the CSV.

📊 Scoring System Explanation

Model Scores

AMP Score: Probability of being an antimicrobial peptide (0-1)

Threshold: 0.998
Score > 0.998 → Predicted as AMP (+1 point)
Based on PeptideBERT-LSTM model trained on validated AMP databases

E.coli Score: Probability of antimicrobial activity against E.coli (0-1)

Threshold: 0.9980798959732056
Score ≥ threshold → Active against E.coli (Gram-negative) (+1 point)
Predicts bactericidal activity against Escherichia coli

S.aureus Score: Probability of antimicrobial activity against S.aureus (0-1)

Threshold: 0.9989857077598572
Score ≥ threshold → Active against S.aureus (Gram-positive) (+1 point)
Predicts bactericidal activity against Staphylococcus aureus

Hemolytic Score: Probability of non-hemolytic activity (0-1), higher is safer

Threshold: 0.5
Score ≥ 0.5 → Non-hemolytic, safe for therapeutic use (+1 point)
Score < 0.5 → Hemolytic, may damage red blood cells
Critical for drug safety assessment

Overall Evaluation

Score: Cumulative score based on all four criteria (0-4 points)

Good Score = 4/4

Meets all criteria: AMP + E.coli activity + S.aureus activity + Non-hemolytic
Broad-spectrum antimicrobial with low toxicity
Highly recommended for further validation and development

Mediocre Score = 2-3/4

Partially meets criteria
May have limited spectrum or safety concerns
Acceptable but requires careful evaluation of trade-offs

Bad Score = 0-1/4

Fails to meet most criteria
Either not antimicrobial, inactive against targets, or toxic
Not recommended for therapeutic development

ℹ️ About Screening: All sequences are evaluated using the same comprehensive 4-criteria system. Results are automatically sorted by score (highest to lowest) to help you identify the most promising candidates quickly.

💡 Note: All submitted sequences remain in the results. Predictions outside a model’s supported length range are N/A, and incomplete evaluations are marked Incomplete.

⚠️ Important: These predictions are computational estimates. High-scoring peptides should be validated experimentally before clinical or commercial use.